Recombinant HSP27 anticorps

N° du produit ABIN7126514

Cet anticorps Souris Monoclonal détecte spécifiquement HSP27 dans WB et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant HSP27 anticorps (ABIN7126514)

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSP27 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-HSP27 anticorps

Specificité: HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSPs and mammalian i±-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human full-length HSP27 protein

Isotype: IgG2a

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or HeLa cells. ~50 % of breast carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur HSP27

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation: HSPB1

Sujet: Heat shock 27 kDa protein, Estrogen-regulated 24 kDa protein, Heat shock 25 kDa protein 1, Heat shock 28 kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27),Heat Shock 27 kDa Protein 1
Cellular localisation: Predominantly cytoplasmic with some nuclear. It is cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub

Poids moléculaire: 27kDa

ID gène: 3315, 520973

UniProt: P04792

Pathways: Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling