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DYKDDDDK Tag anticorps

WB, ELISA, IP, IF Hôte: Souris Monoclonal 4G5C8 unconjugated

Immunoprecipitating Flag Tag in 293F transfected whole cell lysate Lane 1: Mouse control IgG instead of in Flag Tag in 293F transfected whole cell lysate. (DYKDDDDK Tag anticorps)

Western Blot Positive WB detected in: 10 ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa (DYKDDDDK Tag anticorps)

Western Blot Positive WB detected in: Flag Tag fusion protein at 50 ng, 25 ng, 12. (DYKDDDDK Tag anticorps)

Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate Lane 1: Mouse control IgG instead of in Transfected HEK-293 cell whole cell lysate Lane 2: (1 μL) + Transfected HEK-293 cell whole cell lysate (500 μg) Lane 3: Transfected HEK-293 cell whole cell lysate (20 μg) For western blotting, the blot was detected with at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 (DYKDDDDK Tag anticorps)

Western Blot Positive WB detected in: Flag Tag fusion protein1, 2, 3, 4 at 50 ng All lanes: Flag Tag antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 59. (DYKDDDDK Tag anticorps)

Immunofluorescence staining of 293T cells transfected with Flag tag by at 1:300, counter-stained with DAPI. (DYKDDDDK Tag anticorps)

6 images

N° du produit ABIN7322021

Fiche de données

Antigène Tous les produits DYKDDDDK Tag

DYKDDDDK Tag

Reactivité

Veuillez nous consulter SVP
Hôte
57

51

9

8

6
Souris
Anticorps secondaires appropriés
Clonalité
66

64
Monoclonal
Conjugué
46

15

10

7

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp DYKDDDDK Tag est non-conjugé
Application
78

61

28

23

20

19

12

12

10

6

5

4

3

3

3

2

2

2

2

1

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Purification

Protein G purified

Immunogène

DYKDDDDK(Flag) synthetic peptide conjugate to KLH

Clone

4G5C8

Isotype

IgG2a
anti-DYKDDDDK Tag antibody
WB, ELISA, IP Hôte: Lapin Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF Hôte: Souris Monoclonal 2C5 unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IF, FACS Hôte: Lapin Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP, DB, ISt Hôte: Souris Monoclonal FG4R unconjugated

anti-DYKDDDDK Tag antibody
D Hôte: Souris Monoclonal 29E4-G7 unconjugated

anti-DYKDDDDK Tag antibody
Reactivité: Humain WB Hôte: Lapin Polyclonal RB0493 unconjugated

anti-DYKDDDDK Tag antibody (HRP)
WB, ELISA, IP Hôte: Lapin Polyclonal HRP

anti-DYKDDDDK Tag antibody
IP Hôte: Souris Monoclonal 29E4-G7 unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF Hôte: Souris unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP Hôte: Souris Monoclonal 5A8E5 unconjugated

Indications d'application

Recommended dilution: WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5 μL-2 μL,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C,-80 °C

Stockage commentaire

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antigène

DYKDDDDK Tag

Autre désignation

Flag Tag (DYKDDDDK Tag Produits)

Classe de substances

Tag

Sujet

Background: Flag-tag, or Flag octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
Aliases: DDK tag, DYKDDDDK, Flag, Flag Tag

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