TNF alpha anticorps

N° du produit ABIN7140874

Cet anticorps anti-TNF alpha est un anticorps Souris Monoclonal détectant TNF alpha dans ELISA. Adapté pour Boeuf (Vache).

Aperçu rapide pour TNF alpha anticorps (ABIN7140874)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: ELISA

Clone: 2E1A5

Détail du produit anti-TNF alpha anticorps

Fonction: TNF Monoclonal Antibody

Purification: Protein G purified

Pureté: >95 %

Immunogène: Recombinant Bovine TNF alpha protein

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF

Sujet:

Background: Tumor necrosis factor alpha (TNFα,also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNFα is produced by a wide variety of immune and epithelial cell types. Bovine TNFα consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 178 aa extracellular domain (ECD). Within the ECD, bovine TNFα shares 64 % -83 % sequence identity with canine, cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus TNFα.The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness. Cleavage of membrane bound TNFα by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNFα. TNFα trimers bind the ubiquitous TNF RI and the hematopoietic cell restricted TNF RII, both of which are also expressed as homotrimers. TNFα regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNFα is a key cytokine in several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

Aliases: Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2

UniProt: Q06599

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome