POLD2 anticorps (AA 245-430) (Biotin)

N° du produit ABIN7150232

Détail du produit anti-POLD2 anticorps (Biotin)

Antigène: POLD2 (Polymerase (DNA Directed), delta 2, Accessory Subunit (POLD2))

Épitope: AA 245-430

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLD2 est conjugé à/à la Biotin

Application: ELISA

Réactivité croisée: Humain

Purification: >95%, Protein G purified

Immunogène: Recombinant Human DNA polymerase delta subunit 2 protein (245-430AA)

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur POLD2

Antigène: POLD2 (Polymerase (DNA Directed), delta 2, Accessory Subunit (POLD2))

Autre désignation: POLD2 (POLD2 Produits)

Synonymes: anticorps 50kDa, anticorps p50, anticorps po1D2, anticorps cdc1, anticorps si:dz150f13.3, anticorps zgc:55633, anticorps zgc:77043, anticorps DNA polymerase delta 2, accessory subunit, anticorps polymerase (DNA directed), delta 2, regulatory subunit, anticorps polymerase (DNA directed), delta 2, accessory subunit L homeolog, anticorps POLD2, anticorps Pold2, anticorps pold2.L, anticorps pold2

Sujet:

Background: As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair (PubMed:12403614, PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200). Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5\'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS as a component of the POLZ complex. Along with POLD3, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (PubMed:24449906).

Aliases: DNA polymerase delta subunit 2 antibody, DNA polymerase delta subunit p50 antibody, DNA polymerase subunit delta 2 antibody, DNA polymerase subunit delta p50 antibody, DPOD2_HUMAN antibody, POLD 2 antibody, pold2 antibody

UniProt: P49005

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA