LIG4 anticorps

N° du produit ABIN7222216

L’anticorps Lapin Polyclonal anti-LIG4 a été validé pour ELISA et IHC (p). Il convient pour détecter LIG4 dans des échantillons de Humain.

Aperçu rapide pour LIG4 anticorps (ABIN7222216)

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIG4 est non-conjugé

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-LIG4 anticorps

Fonction: DNA Ligase IV Polyclonal Antibody

Specificité: DNA Ligase IV Polyclonal Antibody detects endogenous levels of DNA Ligase IV protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogène: Synthesized peptide derived from human DNA Ligase IV around the non-phosphorylation site of T650

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Commentaires:

Primary Antibody

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Détails sur LIG4

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Autre désignation: DNA Ligase IV

Sujet: Rabbit Anti-DNA Ligase IV Polyclonal Antibody,LIG4, DNA ligase 4, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4,The protein encoded by LIG4 is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.,DNA ligase 4

Poids moléculaire: observerd band 133kDa

ID gène: 3981

UniProt: P49917

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response