PKC zeta anticorps (pThr410)

N° du produit ABIN7213110

L’anticorps anti-PKC zeta Polyclonal Lapin est utilisé pour la détection de PKC zeta dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC, ELISA et IF.

Aperçu rapide pour PKC zeta anticorps (pThr410) (ABIN7213110)

Antigène: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC zeta est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-PKC zeta anticorps

Épitope: pThr410

Fonction: Rabbit Anti-PKC ζ (phospho Thr410) Polyclonal Antibody

Specificité: Phospho-PKC ζ (T410) Polyclonal Antibody detects endogenous levels of PKC ζ protein only when phosphorylated at T410.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human PKC zeta Phospho-Thr410

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur PKC zeta

Antigène: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Autre désignation: PKC zeta

Sujet: PRKCZ, PKC2, Protein kinase C zeta type, nPKC-zetaProtein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.

Poids moléculaire: 70kD

ID gène: 5590

UniProt: Q05513

Pathways: Signalisation NF-kappaB, Signalisation RTK, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling