RAF1 anticorps (pSer642)

N° du produit ABIN7223916

Cet anticorps Lapin Polyclonal détecte spécifiquement RAF1 dans WB et ELISA. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour RAF1 anticorps (pSer642) (ABIN7223916)

Antigène: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAF1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-RAF1 anticorps

Épitope: pSer642

Fonction: Raf-1 (phospho Ser642) Polyclonal Antibody

Specificité: Phospho-Raf-1 (S642) Polyclonal Antibody detects endogenous levels of Raf-1 protein only when phosphorylated at S642.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogène: Synthesized peptide derived from human Raf-1 Phospho-Ser642

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Commentaires:

Primary Antibody

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Détails sur RAF1

Antigène: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Autre désignation: Raf-1

Sujet: Rabbit Anti-Raf-1 (phospho Ser642) Polyclonal Antibody,RAF1, RAF, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1,RAF1 (Raf-1 proto-oncogene, serine/threonine kinase) is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in RAF1 are associated with Noonan syndrome 5 and LEOPARD syndrome 2.,RAF proto-oncogene serine threonine-protein kinase

Poids moléculaire: observerd band 75kDa

ID gène: 5894

UniProt: P04049

Pathways: Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling