Western Blot (1:1,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.,Immunoprecipitation: Does not immunoprecipitate intact proteasomes from HeLa cell preparations.Immunohistochemistry: Has been shown to have applicability in immunohistochemical applications using human tissue.Western blot: Single dimension SDS-PAGE of a HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of ~28 kDa.
Restrictions
For Research Use only
Format
Liquid
Buffer
Liquid. In PBS, pH 7.2, containing 50 % glycerol and 0.09 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid freeze/thaw cycles.
Stock
-20 °C
Stockage commentaire
Store unopened vial at -20°C until required for use. Aliquot undiluted antibody into smaller volumes (not less than 10μl) prior to freezing if appropriate. The use of high quality 'antiserum-grade' plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month.