IL-1 beta anticorps (C-Term)

N° du produit ABIN7464948

Cet anticorps Souris Monoclonal détecte spécifiquement IL-1 beta dans WB, ICC et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour IL-1 beta anticorps (C-Term) (ABIN7464948)

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-1 beta est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: GT289

Détail du produit anti-IL-1 beta anticorps

Épitope: C-Term

Réactivité croisée: Humain

Purification: Affinity purified by Protein A.

Immunogène: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IL1 beta. The exact sequence is proprietary.

Isotype: IgG2a

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: THP-1 (500 ng/mL LPS treatment for 6 hr and then 300 ng/mL Brefeldin treatment for the last 3 hr)

Validation: Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur IL-1 beta

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: interleukin 1 beta

Sujet: Interleukin 1 beta , IL-1 , IL1-BETA , IL1F2,The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq]

Poids moléculaire: 31 kDa

ID gène: 3553

UniProt: P01584

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome