ATR anticorps (C-Term)

N° du produit ABIN7467983

Cet anticorps anti-ATR est un anticorps Lapin Polyclonal détectant ATR dans WB, IP, ICC, IHC (p) et IF. Adapté pour Humain.

Aperçu rapide pour ATR anticorps (C-Term) (ABIN7467983)

Antigène: ATR (Ataxia Telangiectasia and Rad3 Related (ATR))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATR est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Classe de qualité: KO Validated

Détail du produit anti-ATR anticorps

Épitope: C-Term

Réactivité croisée: Humain, Souris

Purification: Purified by antigen-affinity chromatography.

Immunogène: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATR. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:5000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: 293T , A431 , HeLa , HepG2 , Raji nuclear extract Validation: Comparison, KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur ATR

Antigène: ATR (Ataxia Telangiectasia and Rad3 Related (ATR))

Autre désignation: ATR serine/threonine kinase

Sujet:

Synonyms: ATR serine/threonine kinase , FCTCS , FRP1 , MEC1 , SCKL , SCKL1

Background: The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq]

Poids moléculaire: 301 kDa

ID gène: 545

UniProt: Q13535

Pathways: Signalisation p53, Apoptose, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus