ATR anticorps (pThr1989)

N° du produit ABIN7467984

Cet anticorps Souris Monoclonal détecte spécifiquement ATR dans WB, IP, ICC et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour ATR anticorps (pThr1989) (ABIN7467984)

Antigène: ATR (Ataxia Telangiectasia and Rad3 Related (ATR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ATR est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: GT222

Détail du produit anti-ATR anticorps

Épitope: pThr1989

Réactivité croisée: Humain

Purification: Affinity purified by Protein A.

Immunogène: Carrier-protein conjugated synthetic peptide surrounding phospho Thr1989 of human ATR. The exact sequence is proprietary.

Isotype: IgG2b

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: HCT116 , HCT116(100 J/m2 UVC and recover for 6hr) Validation: Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur ATR

Antigène: ATR (Ataxia Telangiectasia and Rad3 Related (ATR))

Autre désignation: ATR serine/threonine kinase

Sujet:

Synonyms: ATR serine/threonine kinase , FCTCS , FRP1 , MEC1 , SCKL , SCKL1

Background: The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq]

Poids moléculaire: 301 kDa

ID gène: 545

UniProt: Q13535

Pathways: Signalisation p53, Apoptose, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus