BMI1 Proto-Oncogene, Polycomb Ring Finger (BMI1) anticorps

N° du produit ABIN7468056

Détail du produit

Antigène: BMI1 Proto-Oncogene, Polycomb Ring Finger (BMI1)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Réactivité croisée: Cat, Chien, Humain, Souris

Purification: Purified by antigen-affinity chromatography.

Classe de qualité: KO Validated

Immunogène: Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: THP-1 , HL-60 , C8D30 , BCL-1 , Raw264.7 , K562 , THP-1 , 293T , A549 , H1299 , HCT116 Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détail du antigène

Antigène: BMI1 Proto-Oncogene, Polycomb Ring Finger (BMI1)

Autre désignation: BMI1 proto-oncogene, polycomb ring finger

Synonymes: anticorps FLVI2/BMI1, anticorps PCGF4, anticorps RNF51, anticorps BMI1 proto-oncogene, polycomb ring finger, anticorps BMI1

Sujet:

Synonyms: BMI1 proto-oncogene, polycomb ring finger , FLVI2/BMI1 , PCGF4 , RNF51 , flvi-2/bmi-1

Background: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Poids moléculaire: 37 kDa

ID gène: 648

UniProt: P35226