Recombinant p21 anticorps (AA 120-138)

N° du produit ABIN7487750

Cet anticorps Rat Monoclonal détecte spécifiquement p21 dans IP. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour Recombinant p21 anticorps (AA 120-138) (ABIN7487750)

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp p21 est non-conjugé

Application: Immunoprecipitation (IP)

Clone: Y13-238

Détail du produit anti-p21 anticorps

Épitope: AA 120-138

Fonction: Anti-p21 [Y13-238], Rat IgG2a, lambda

Specificité:

The antibody reacts with v-H-ras (within amino acids 120-138) and rodent c-H-ras p21's but not v-K-ras or rodent c-K-ras p21's. This antibody also recognizes human c-H-ras and c-K-ras p21's, but not human c-N-ras.

Application Notes original Clone: Y13-238 has been used to immunoprecipitate p21 from human peripheral blood lymphoblasts in order to measure its activation state (Downward 1990). Y13-238 has been used as an anti-ras non-neutralising scFv (Cochet 1998).

Réactivité croisée: Souris

Purification: Protein A affinity purified

Immunogène: Injection of rats subcutaneously with Ha-MuSV transformed NRK cells and screening of sera of individual animals to identify those with the strongest immune response against p21. Spleens were removed from the immunised animals and cell fusion of immune lymphocytes with rat myeloma cell line 210RCY3 Ag 1.2.3 (Y3) was carried out. Supernatants were harvested and assayed for antibodies to p21 by GDP binding assay or immunofluorescence on Ha-SV-transformed MDCK cells, followed by testing for the ability of the antibodies to immunoprecipitate p21.

Isotype: IgG2a, lambda

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Rat

OriginalFormat: IgG2a

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur p21

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Autre désignation: p21

Sujet: Ras, p21ras,

UniProt: P78460, O70564

Pathways: Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy