ERN1 anticorps (Internal Region)

N° du produit ABIN7540596

N° du produit (Fournisseur): 600-401-hb9s

L’anticorps Lapin Polyclonal anti-ERN1 a été validé pour WB, ELISA, IHC, IF et FM. Il convient pour détecter ERN1 dans des échantillons de Humain.

Aperçu rapide pour ERN1 anticorps (Internal Region) (ABIN7540596)

Antigène: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERN1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-ERN1 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-hb9s

Fournisseur: Rockland

Fonction: Ire1 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against mouse Ire1.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène: Anti-Ire1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Ire1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:100

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-Ire1 Antibody has been tested in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry. Expect a band in mouse for isoform 1 at ~110.2kDa and 2 at ~45.3kDa, and in human at ~109.7 kDa in western blot using appropriate lysates or tissues.  Positive control used: Mouse brain tissue in Western Blot,  SCLC-21H cells in IF, and Human stomach tissue in IHC.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur ERN1

Antigène: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Autre désignation: Ern1

Sujet: Rabbit Anti-Ern1 Antibody, Rabbit Anti-Endoplasmic Reticulum To Nucleus Signaling 1 Antibody, Rabbit Anti-IRE1 Antibody, Endoplasmic Reticulum To Nucleus Signaling 1, Serine/Threonine-Protein Kinase/Endoribonuclease IRE1, Inositol-Requiring Protein 1, Inositol-Requiring Enzyme 1, ER To Nucleus Signalling 1, Ire1-Alpha, HIRE1p, IRE1a, IRE1, Endoplasmic Reticulum-To-Nucleus Signaling 1, Protein Kinase/Endoribonuclease, Inositol-Requiring 1, IRE1P,Inositol-requiring enzyme 1 (IRE1) or ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1) is a Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain, and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes. Diseases associated with ERN1 include Chromosome 6Pter-P24 Deletion Syndrome and Alzheimer Disease. Anti-IRE1 Antibody is useful for researchers interested in Neuroscience research.

ID gène: 78943

NCBI Accession: NP_076402

UniProt: Q9EQY0

Pathways: ER-Nucleus Signaling, Unfolded Protein Response