MEK1 anticorps (C-Term)

N° du produit ABIN7565794

Cet anticorps Souris Monoclonal détecte spécifiquement MEK1 dans WB et ELISA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour MEK1 anticorps (C-Term) (ABIN7565794)

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MEK1 est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: 13B6-G12

Détail du produit anti-MEK1 anticorps

Épitope: C-Term

Fonction: MEK1 C-Term Antibody

Réactivité croisée (Details): This protein A purified mouse monoclonal antibody reacts specifically with human MEK1.

Attributs du produit: Synonyms: mouse anti-MEK1 antibody, MAP2K, MEK, MEK 1, MKK1, PRKMK1, MEK-1 Antibody

Purification: Anti-MEK1 is purified from tissue culture supernatant by protein A purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-MEK1 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application: Application Note: Anti-MEK 1 (MOUSE) antibody is tested in ELISA and Western Blotting.  Specific conditions of reactivity should be optimized by the end user.  Expect a band of approximately 43.5 kDa. Western Blot Dilution: 1 μg/mL ELISA Dilution: 1:40,000 Other: PAGE-MAP/IP-MS: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur MEK1

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Autre désignation: MAP2K1

Sujet: Background: MEK1 antibodies detect the MEK1 isoform.  Mitogen-activated protein kinase kinase 1, also known as MKK or MEK1, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation.   This pathway also plays a key role in synaptic plasticity in the brain.  Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase.   MEK1 and MEK2 are about 80 % identical to each other, and nearly identical within the kinase domain.   The MEK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

UniProt: Q02750

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling