TNF alpha anticorps

N° du produit ABIN7565961

Cet anticorps anti-TNF alpha Polyclonal Lapin (ABIN7565961) détecte spécifiquement TNF alpha dans WB, IHC et ELISA. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour TNF alpha anticorps (ABIN7565961)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-TNF alpha anticorps

Fonction: TNF alpha Antibody

Réactivité croisée (Details): The antiserum is directed against mature 17,000 MW mouse TNFa and is useful in determining its presence in various assays.

Attributs du produit: Synonyms: rabbit anti-Mouse TNF alpha Antibody, APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody

Purification: This antiserum has been heated to 56°C for 30 minutes.

Stérilité: Sterile filtered

Immunogène:

Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant mouse TNFa produced in E.coli.

Immunogen Type: Recombinant Protein

Information d'application

Indications d'application: Application Note: This antiserum against Mouse TNFa has been tested for use in immunoblotting.  This antibody is suitable for use in neutralizations, ELISA, radioimmunoassay, immunoprecipitation, and immunohistochemistry. Reactivity in other immunoassays is unknown. It recognizes the 17,000 MW TNFa. This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples.  This antibody is also useful for neutralization of mouse activity in bioassays.  It does not neutralize the biological activity of lymphotoxin.  For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG is recommended. Immunohistochemistry Dilution: 1:100 - 1:500 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 85 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: None

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tnf

Sujet: Background: TNF alpha Antibody detects TNF-a protein. TNF-a is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is a potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Anti-TNF-alpha Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.

ID gène: 21926

UniProt: P06804

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome