LIG4 anticorps (AA 1-876)

N° du produit ABIN7599567

Cet anticorps anti-LIG4 est un anticorps Lapin Polyclonal détectant LIG4 dans WB, ELISA, IF, ICC et FACS. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour LIG4 anticorps (AA 1-876) (ABIN7599567)

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIG4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-LIG4 anticorps

Épitope: AA 1-876

Fonction: Anti-DNA Ligase IV/LIG4 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-DNA Ligase IV/LIG4 Antibody Picoband® (ABIN7599567). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human DNA Ligase IV/LIG4 recombinant protein (Position: M1-K876).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ben-Omran, T. I., Cerosaletti, K., Concannon, P., Weitzman, S., Nezarati, M. M. A patient with mutations in DNA ligase IV: clinical features and overlap with Nijmegen breakage syndrome. Am. J. Med. Genet. 137A: 283-287, 2005. 2. Frank, K. M., Sekiguchi, J. M., Seidl, K. J., Swat, W., Rathbun, G. A., Cheng, H.-L., Davidson, L., Kangaloo, L., Alt, F. W. Late embryonic lethality and impaired V(D)J recombination in mice lacking DNA ligase IV. Nature 396: 173-177, 1998. 3. Frank, K. M., Sharpless, N. E., Gao, Y., Sekiguchi, J. M., Ferguson, D. O., Zhu, C., Manis, J. P., Horner, J., DePinho, R. A., Alt, F. W. DNA ligase IV deficiency in mice leads to defective neurogenesis and embryonic lethality via the p53 pathway. Molec. Cell 5: 993-1002, 2000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur LIG4

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Autre désignation: LIG4

Sujet:

Synonyms: Cell surface glycoprotein MUC18, Gicerin, Melanoma cell adhesion molecule, Melanoma-associated antigen MUC18, CD146, Mcam, Muc18

Tissue Specificity: Detected in melanoma cell lines.

Background: DNA ligase 4 is an enzyme that in humans is encoded by the LIG4 gene. The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Poids moléculaire: 104 kDa

ID gène: 3981

UniProt: P49917

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response