SPHK1 anticorps (AA 55-357)

N° du produit ABIN7602016

L’anticorps Lapin Polyclonal anti-SPHK1 a été validé pour WB, ELISA et FACS. Il convient pour détecter SPHK1 dans des échantillons de Humain.

Aperçu rapide pour SPHK1 anticorps (AA 55-357) (ABIN7602016)

Antigène: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SPHK1 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-SPHK1 anticorps

Épitope: AA 55-357

Fonction: Anti-SPHK1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-SPHK1 Antibody Picoband® (ABIN7602016). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human SPHK1 recombinant protein (Position: E55-N357).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kohama, T., Olivera, A., Edsall, L., Nagiec, M. M., Dickson, R., Spiegel, S. Molecular cloning and functional characterization of murine sphingosine kinase. J. Biol. Chem. 273: 23722-23728, 1998. 2. Melendez, A. J., Carlos-Dias, E., Gosink, M., Allen, J. M., Takacs, L. Human sphingosine kinase: molecular cloning, functional characterization and tissue distribution. Gene 251: 19-26, 2000. 3. Pitson, S. M., D'Andrea, R. J., Vandeleur, L., Moretti, P. A. B., Xia, P., Gamble, J. R., Vadas, M. A., Wattenberg, B. W. Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes. Biochem. J. 350: 429-441, 2000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur SPHK1

Antigène: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Autre désignation: SPHK1

Sujet:

Synonyms: Hexokinase-2,2.7.1.1,Hexokinase type II,HK II,Muscle form hexokinase,HK2,

Tissue Specificity: Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.

Background: SPHK1(Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.

Poids moléculaire: 52 kDa

ID gène: 8877

Pathways: VEGF Signaling