ZAP70 anticorps (Middle Region)

N° du produit ABIN7602981

Cet anticorps Souris Monoclonal détecte spécifiquement ZAP70 dans WB et FACS. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour ZAP70 anticorps (Middle Region) (ABIN7602981)

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ZAP70 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 9D5

Détail du produit anti-ZAP70 anticorps

Épitope: Middle Region

Fonction: Anti-ZAP70 Antibody Picoband® (monoclonal, 9D5)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-ZAP70 Antibody Picoband® (monoclonal, 9D5) (ABIN7602981). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human ZAP70, different from the related mouse sequence by two amino acids.

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Lupher ML, Reedquist KA, Miyake S, Langdon WY, Band H (Sep 1996). "A novel phosphotyrosine-binding domain in the N-terminal transforming region of Cbl interacts ly and selectively with ZAP-70 in T cells".The Journal of Biological Chemistry 271 (39): 24063-8. 2. Meng W, Sawasdikosol S, Burakoff SJ, Eck MJ (Mar 1999). "Structure of the amino-terminal domain of Cbl complexed to its binding site on ZAP-70 kinase". Nature 398 (6722): 84-90. 3. Neumeister EN, Zhu Y, Richard S, Terhorst C, Chan AC, Shaw AS (Jun 1995). "Binding of ZAP-70 to phosphorylated T-cell receptor zeta and eta enhances its autophosphorylation and generates specific binding sites for SH2 domain-containing proteins". Molecular and Cellular Biology 15 (6): 3171-8.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur ZAP70

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Autre désignation: ZAP70

Sujet:

Synonyms: SRK, STD, TZK, ZAP70, ZAP-70, ZAP 70, P43403

Tissue Specificity: Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.

Background: ZAP-70 (Zeta-chain-associated protein kinase 70) encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Poids moléculaire: 72 kDa

ID gène: 7535

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway