HMGB1 anticorps

N° du produit ABIN7670629

Cet anticorps anti-HMGB1 est un anticorps Lapin Polyclonal détectant HMGB1 dans WB et ELISA. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour HMGB1 anticorps (ABIN7670629)

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HMGB1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-HMGB1 anticorps

Fonction: HMGB-1 Polyclonal Antibody(Capture/Detector)

Purification: Antigen Affinity Purification

Immunogène: Recombinant Human HMGB-1 protein expressed by E.coli

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:1000,ELISA Capture 2-8 μg/mL,ELISA Detector 0.1-0.4 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Sterile PBS , pH 7.4

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C Valid for 1 month or -20°C Valid for 12 months

Date de péremption: 12 months

Détails sur HMGB1

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Autre désignation: HMGB-1

Sujet: HMGB1,HMG1,High mobiLity group protein B1,High mobiLity group protein 1 (HMG-1),Human High-mobility group box 1 protein (HMGB1),previously known as HMG-1 or amphoterin,is a member of the high mobility group box family of non-histone chromosomal proteins.HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly,both as an inflammatory mediator that promotes monocyte migration and cytokine secretion,and as a mediator of T cell-dendritic cell interaction. The cytokine activity of HBMG1 is restricted to the HMG B box,while the A box is associated with the helix-loop-helix domain of transcription factors. HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence,it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted,HMGB1 transduces cellular signals through its high affinity receptor,RAGE and,possibly,TlR2 and TlR4. Human HMGB1 is 100 % aa identical to canine HMGB1 and 99 % aa identical to mouse,rat,bovine and porcine HMGB1,respectively.

Poids moléculaire:

Calculated MW: 29kDa

ObservedMW: 29kDa

UniProt: P09429

Pathways: Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome