Recombinant CD3 epsilon anticorps

N° du produit ABIN7795311

L’anticorps Souris Monoclonal anti-CD3 epsilon a été validé pour ELISA, Func, Neut et IGA. Il convient pour détecter CD3 epsilon dans des échantillons de Humain.

Aperçu rapide pour Recombinant CD3 epsilon anticorps (ABIN7795311)

Antigène: CD3 epsilon (CD3E)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: ELISA, Functional Studies (Func), Neutralization (Neut), Immunogenicity Assay (IGA)

Classe de qualité: In vivo Grade

Clone: OKT3

Détail du produit anti-CD3 epsilon anticorps

Expression System: Mammalian cells

Fonction: In vivo Grade Recombinant Anti-human CD3e Monoclonal Antibody, Mouse IgG2a Kappa (Clone: OKT3)

Attributs du produit: The OKT3 antibody binds to CD3 epsilon of the T cell receptor-CD3 complex found only on mature T cells and medullary thymocytes. An interaction between T cells, OKT3, and monocytes causes T-cell activation (mitogenesis) in vitro. On the other hand, the OKT3 antibody blocks the unique association between the antigen receptor and CD3, and both the generation and function of cytotoxic T cells. After an initial dose of the OKT3 antibody, T cells disappear from the circulation within minutes to hours. During treatment with the OKT3 antibody, T cells bearing the usual array of surface molecules (CD2, CD4, and CD8) reappear in the peripheral blood circulation but these cells are devoid of CD3. After another 48 hours without the OKT3 antibody, the normal array of surface molecules including CD3 appear again on all T cells. The selective removal of CD3 by internalization is thought to be the key mechanism of action of the OKT3 antibody. Comodulation of the antigen receptor with CD3 explains the immunoblocking action of the OKT3 antibody in vivo. The OKT3 antibody is ineffective clinically if the CD3 target molecule is not modulated. The OKT3 antibody is effective for induction of immunosuppression and for treating initial and steroid-resistant rejections.

Purification: Protein A or G affinity column

Pureté: >95 % by reducing SDS-PAGE

niveau d'endotoxine: < 1 EU per 1 mg of the protein by the LAL method

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: > 3 mg/mL

Stock: 4 °C,-20 °C

Stockage commentaire: Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3e

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway