Recombinant CD3 epsilon anticorps

N° du produit ABIN7795319

Cet anticorps Human Monoclonal détecte spécifiquement CD3 epsilon dans FACS. Il présente une réactivité envers Souris.

Aperçu rapide pour Recombinant CD3 epsilon anticorps (ABIN7795319)

Antigène: CD3 epsilon (CD3E)

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Human

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Flow Cytometry (FACS)

Clone: 145-2C11

Détail du produit anti-CD3 epsilon anticorps

Expression System: Mammalian cells

Fonction: Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11), Human IgG1 Fc Silent Kappa

Attributs du produit: The antibody binds to CD3 epsilon of the T cell receptor-CD3 complex found only on mature T cells and medullary thymocytes. An interaction between T cells, the antibody, and monocytes causes T-cell activation (mitogenesis) in vitro. On the other hand, the antibody blocks the unique association between the antigen receptor and CD3, and both the generation and function of cytotoxic T cells. After an initial dose of the antibody, T cells disappear from the circulation within minutes to hours. During treatment with the antibody, T cells bearing the usual array of surface molecules (CD2, CD4, and CD8) reappear in the peripheral blood circulation but these cells are devoid of CD3. After another 48 hours without the antibody, the normal array of surface molecules including CD3 appear again on all T cells. The selective removal of CD3 by internalization is thought to be the key mechanism of action of the antibody. Comodulation of the antigen receptor with CD3 explains the immunoblocking action of the antibody in vivo. The antibody is ineffective clinically if the CD3 target molecule is not modulated. The antibody is effective for induction of immunosuppression and for treating initial and steroid-resistant rejections.

Purification: Protein A or G affinity column

Pureté: >95 % by reducing SDS-PAGE

Isotype: Fc Silenced, IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: > 3 mg/mL

Stock: 4 °C,-20 °C

Stockage commentaire: Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3e

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway