Recombinant ZAP70 anticorps (AA 247-382)
Aperçu rapide pour Recombinant ZAP70 anticorps (AA 247-382) (ABIN7826139)
Antigène
Voir toutes ZAP70 AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 247-382
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Fonction
- ZAP-70 antibody (870R)
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Immunogène
- Recombinant fragment (around aa 247-382) ofhuman ZAP70 protein (exact sequence is proprietary)
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Isotype
- IgG
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Indications d'application
- IHC 1:75-1:150
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Commentaires
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Positive Control: Tonsil: A large fraction of the lymphocytes in the interfollicular areas and few lymphocytes in the germinal centres should show a strong ZAP70 immunostaining.
Product Description/Background: ZAP-70 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Tonsil: ZAP70 immunostaining should be absent in epithelial tissues and the majority of cells in germinal centres.
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Protocole
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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Date de péremption
- 24 months
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- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
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Autre désignation
- ZAP-70
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Sujet
- Gene Alias: Selective T cell defect, SRK, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70,TZK, Zeta chain associated protein kinase 70 kDa
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UniProt
- P43403
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Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
Antigène
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