Integrin Alpha2b anticorps (Biotin)

N° du produit ABIN7836294

Cet anticorps Souris Monoclonal détecte spécifiquement Integrin Alpha2b dans FACS et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Integrin Alpha2b anticorps (Biotin) (ABIN7836294)

Antigène: Integrin Alpha2b (CD41)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Integrin Alpha2b est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HIP8

Détail du produit anti-Integrin Alpha2b anticorps (Biotin)

Fonction: Mouse monoclonal antibody Biotin labled to CD41

Specificité: Recognizes human CD41

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD41.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature. 7. Repeat step 5. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur Integrin Alpha2b

Antigène: Integrin Alpha2b (CD41)

Autre désignation: ITGA2B

Sujet: GP2B, ITGAB, Integrin alpha-IIb, GPalpha IIb, GPIIb, Platelet membrane glycoprotein IIb, CD41

ID gène: 3674

UniProt: P08514

Pathways: Integrin Complex