HLA-DRA anticorps (FITC)

N° du produit ABIN7839676

L’anticorps anti-HLA-DRA Monoclonal Souris est utilisé pour la détection de HLA-DRA dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour HLA-DRA anticorps (FITC) (ABIN7839676)

Antigène: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HLA-DRA est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: LN3

Détail du produit anti-HLA-DRA anticorps (FITC)

Fonction: Mouse monoclonal antibody FITC labled to HLA-DR

Specificité: Recognizes human HLA-DR

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human HLA-DR.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur HLA-DRA

Antigène: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Autre désignation: HLA-DRA

Sujet: HLA-DRA1, HLA class II histocompatibility antigen. DR alpha chain, MHC class II antigen DRA

ID gène: 3122

UniProt: P01903

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Human Leukocyte Antigen (HLA) in Adaptive Immune Response