FOXO1 anticorps (AA 450-650)

N° du produit ABIN7860174

L’anticorps anti-FOXO1 Monoclonal Souris est utilisé pour la détection de FOXO1 dans des échantillons de Humain. Il a été validé pour WB et IHC.

Aperçu rapide pour FOXO1 anticorps (AA 450-650) (ABIN7860174)

Antigène: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FOXO1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: FOXO1-9792

Détail du produit anti-FOXO1 anticorps

Épitope: AA 450-650

Fonction: FOXO1 (Forkhead box O1) (Transcription Factor) Antibody

Specificité: FKHR (for forkhead in rhabdomyosarcoma) and FKHRL1 are members of the forkhead family of transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 and results in FKHRL1 associating with 14-3-3 proteins and being retained in the cytoplasm. Induction of apoptosis or withdrawal of growth factors stimulates dephosphorylation and nuclear translocation of FKHR proteins, leading to FKHR-induced gene-specific transcriptional activation. FKHR, also designated forkhead box protein O1A (FOXO1), is a ubiquitously expressed protein that shuttles between the cytoplasm and nucleus. Genetic mutations in FKHR genes, including the t(2,13) and t(1,3) translocations, are commonly found in alveolar rhabdomyosarcomas. These translocations result in the fusion of the amino terminus of Pax-3 or Pax-7, including the paired box and homeodomain DNA-binding domains, with the carboxyterminus of FKHR, which contains a transcriptional activation domain. The Pax-3/FKHR fusion protein appears to function as an oncogenic transcription factor that enhances the activation of normal Pax-3 target genes.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment (around aa450-650) of human FOXO1 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur FOXO1

Antigène: FOXO1 (Forkhead Box O1 (FOXO1))

Autre désignation: FOXO1

Sujet:

forkhead box protein O1A forkhead, Drosophila, homolog of, in rhabdomyosarcoma

Human brain, skeletal muscle, ovary or prostate.

Poids moléculaire: 80kDa

ID gène: 2308

UniProt: Q12778

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling