p21 anticorps (pSer146) (HRP)

N° du produit ABIN756901

Cet anticorps Lapin Polyclonal détecte spécifiquement p21 dans ELISA, IHC (p) et IHC (fro). Il présente une réactivité envers Humain.

Aperçu rapide pour p21 anticorps (pSer146) (HRP) (ABIN756901)

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp p21 est conjugé à/à la HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-p21 anticorps (HRP)

Épitope: pSer146

Réactivité croisée: Humain

Homologie: Dog,Cow,Sheep,Pig,Guinea Pig

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human CDKN1A around the phosphorylation site of Ser146 [QT(p-S)MT]

Isotype: IgG

Information d'application

Indications d'application: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur p21

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Autre désignation: CDKN1A

Sujet:

Synonyms: Activating Fragment 1, CAP20, Cation chloride cotransporter-interacting protein 1, CDK Interacting Protein 1, CDK-interacting protein 1, CDKI, CDKN 1, CDKN1, CDKN1A, CIP1, Cyclin Dependent Kinase Inhibitor 1A, Cyclin-dependent kinase inhibitor 1, Cyclin-dependent kinase inhibitor 1A P21, Cyclin-dependent kinase inhibitor 1A p21, Cip1, DNA Synthesis Inhibitor, MDA 6, MDA-6, MDA6, Melanoma Dferentiation Associated Protein 6, Melanoma dferentiation-associated protein 6, Melanoma dferentiation-associated protein, p21, P21 protein, p21CIP1, p21WAF, PIC1, SDI1, SLC12A9, WAF1, Wildtype p53 Activating Fragment 1, Wildtype p53-activated fragment 1.

Background: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. Two families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically.

ID gène: 1026

Pathways: Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy