Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (AA 101-200) anticorps (HRP)

N° du produit ABIN760265

Détail du produit

Antigène: Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB)

Épitope: AA 101-200

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Humain

Homologie: Mouse,Rat,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human RNF56/CBL-B

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB)

Autre désignation: RNF56 (CBLB Produits)

Synonymes: anticorps rnf56, anticorps cblb, anticorps AI429560, anticorps AI851073, anticorps Cbl-b, anticorps Cbl proto-oncogene B, E3 ubiquitin protein ligase S homeolog, anticorps Cbl proto-oncogene B, E3 ubiquitin protein ligase L homeolog, anticorps Casitas B-lineage lymphoma b, anticorps Cbl proto-oncogene B, anticorps cblb.S, anticorps cblb.L, anticorps Cblb, anticorps CBLB

Sujet:

Synonyms: Cbl-b, RNF56, Nbla00127, E3 ubiquitin-protein ligase CBL-B, Casitas B-lineage lymphoma proto-oncogene b, RING finger protein 56, SH3-binding protein CBL-B, Signal transduction protein CBL-B, CBLB

Background: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.

ID gène: 868

UniProt: Q13191