RAF1 anticorps (AA 641-645)
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- Antigène Voir toutes RAF1 Anticorps
- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
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Épitope
- AA 641-645
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Reactivité
- Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp RAF1 est non-conjugé
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Application
- Western Blotting (WB)
- Specificité
- The antibody detects endogenous level of total Raf-1 protein.
- Purification
- Affinity purified
- Immunogène
- Peptide sequence around AA 641-645 (T-S-P-R-L ) derived from Rat Raf-1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
- Top Product
- Discover our top product RAF1 Anticorps primaire
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- Indications d'application
- Western blotting: 1:500-1:1000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- Antigène
- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
- Autre désignation
- Raf-1 (RAF1 Produits)
- Synonymes
- anticorps CRAF, anticorps NS5, anticorps Raf-1, anticorps c-Raf, anticorps c-raf, anticorps craf, anticorps raf1, anticorps RAF1, anticorps 6430402F14Rik, anticorps AA990557, anticorps BB129353, anticorps Craf1, anticorps D830050J10Rik, anticorps v-Raf, anticorps raf, anticorps cRaf, anticorps Raf-1 proto-oncogene, serine/threonine kinase, anticorps Raf-1 proto-oncogene, serine/threonine kinase a, anticorps v-raf-leukemia viral oncogene 1, anticorps Raf-1 proto-oncogene, serine/threonine kinase S homeolog, anticorps RAF1, anticorps Raf1, anticorps raf1a, anticorps raf1, anticorps raf1.S
- Sujet
- A-Raf, B-Raf and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497 and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338 and the Src family phosphorylates Tyr341 to induce c-Raf activity (3,4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6,7). While A-Raf, B-Raf and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428 and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8,9). The B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301 and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to Avruch,
- Poids moléculaire
- 74 kDa
- ID gène
- 24703
- NCBI Accession
- NP_036771
- UniProt
- P11345
- Pathways
- Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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