TNF alpha anticorps

N° du produit ABIN872451

Cet anticorps anti-TNF alpha est un anticorps Lapin Polyclonal détectant TNF alpha dans WB, IHC (p) et IF (p). Adapté pour Humain, Porc et Chien. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour TNF alpha anticorps (ABIN872451)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain, Porc, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Détail du produit anti-TNF alpha anticorps

Réactivité croisée: Chien, Humain, Porc

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from dog TNF-alpha

Isotype: IgG

Information d'application

Indications d'application: WB (1:100-500),IHC-P (1:100-500),IF (p) (1:100-500)

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Références pour anticorps anti-TNF alpha (ABIN872451)

Zhang, Liu, Hu, Jiang, Zhu: ""Proof-of-principle" concept for ultrasensitive detection of cytokines based on the electrically heated carbon paste electrode." dans: Chemical communications (Cambridge, England), Vol. 47, Issue 23, pp. 6551-3, (2011) (PubMed).

Liao, Zhou, Ji, Zhang: "Eccentric contraction induces inflammatory responses in rat skeletal muscle: role of tumor necrosis factor-alpha." dans: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 298, Issue 3, pp. R599-607, (2010) (PubMed).

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF-alpha

Sujet:

Synonyms: APC1, Cachectin, D, Dferentiation inducing factor, Macrophage cytotoxic factor, MCF, Necrosin, TNF a, TNF alpha, TNF, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor, Tumour Necrosis Factor Alpha, TNFA_HUMAN.

Background: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Poids moléculaire: 17/26kDa

ID gène: 403922

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome