PKMYT1 anticorps (pThr495) (Alexa Fluor 555)

N° du produit ABIN878287

Détail du produit anti-PKMYT1 anticorps (Alexa Fluor 555)

Antigène: PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))

Épitope: pThr495

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKMYT1 est conjugé à/à la Alexa Fluor 555

Application: Western Blotting (WB)

Réactivité croisée: Humain, Souris

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human PKMYT1 around the phosphorylation site of Thr495

Isotype: IgG

Information d'application

Indications d'application: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur PKMYT1

Antigène: PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))

Autre désignation: Pkmyt1 (PKMYT1 Produits)

Synonymes: anticorps MYT1, anticorps 6230424P17, anticorps AW209059, anticorps Myt1, anticorps RGD1305434, anticorps myt1, anticorps protein kinase, membrane associated tyrosine/threonine 1, anticorps protein kinase, membrane associated tyrosine/threonine 1 S homeolog, anticorps PKMYT1, anticorps pkmyt1, anticorps Pkmyt1, anticorps pkmyt1.S

Sujet:

Synonyms: Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Background: Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1.

ID gène: 2475

Pathways: Mitotic G1-G1/S Phases, M Phase