T7-Tag anticorps

N° du produit ABIN920810

Détail du produit anti-T7-Tag anticorps

Antigène: T7-Tag

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp T7-Tag est non-conjugé

Application: Western Blotting (WB), ELISA

Séquence: MASMTGGQQM G

Purification: Affinity purified

Information d'application

Indications d'application: Western blot: Use at 1:5,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS, pH 7.2 and 0.1% sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur T7-Tag

Antigène: T7-Tag

Autre désignation: T7 tag (T7-Tag Produits)

Classe de substances: Tag

Sujet: Rabbits were immunized with MASMTGGQQMG (T7 epitope) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity-purified using the peptide conjugated to a solid-phase support. Specification: The affinity-purified antibodies were analyzed using ELISA and Western blot analyses. The anti-T7 diluted 1:35,000 gave an OD = 1.0 in a 15 minute reaction against peptide conjugated with a different carrier than was used for purification. HRP-conjugated donkey anti-rabbit IgG was used and TMB was the substrate.