alpha Tubulin anticorps (AA 427-441) (HRP)
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- Antigène Voir toutes alpha Tubulin (TUBA1) Anticorps
- alpha Tubulin (TUBA1)
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Épitope
- AA 427-441
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Reactivité
- Humain, Souris, Rat, Poulet, Boeuf (Vache), Chévre, Mouton
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp alpha Tubulin est conjugé à/à la HRP
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Application
- Western Blotting (WB), ELISA
- Specificité
- Anti-Tubulin is directed against human alpha Tubulin protein. The Tubulin Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
- Réactivité croisée
- Souris, Rat (Rattus), Poulet, Mouton, Chévre
- Attributs du produit
- Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
- Stérilité
- Sterile filtered
- Immunogène
- Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin.
- Isotype
- IgG
- Top Product
- Discover our top product TUBA1 Anticorps primaire
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- Indications d'application
- Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.
- Commentaires
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Gene Name: TUBA1B
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Agent conservateur
- Gentamicin sulfate
- Conseil sur la manipulation
- Do NOT add Sodium Azide!
- Stock
- 4 °C
- Stockage commentaire
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening.
- Date de péremption
- 12 months
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- Antigène
- alpha Tubulin (TUBA1)
- Autre désignation
- Alpha-Tubulin (TUBA1 Produits)
- Synonymes
- anticorps Tuba1, anticorps K-ALPHA-1, anticorps Tuba-1, anticorps cb944, anticorps fb22g06, anticorps tuba1, anticorps wu:fb22g06, anticorps Calpha1, anticorps tubulin, alpha 1A, anticorps tubulin alpha 1b, anticorps tubulin, alpha 1b, anticorps tubulin, alpha 1B, anticorps Tuba1a, anticorps TUBA1B, anticorps tuba1b, anticorps Tuba1b
- Sujet
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Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
Synonyms: HRP, Loading Control Antibody - Pathways
- Dynamique des Microtubules
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