MYB anticorps (N-Term, pThr486)

N° du produit ABIN964595

Détail du produit anti-MYB anticorps

Antigène: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Épitope: N-Term, pThr486

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MYB est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: This affinity-purified antibody is directed against the phosphorylated form of mouse c-Myb protein at the pT486 residue. Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.  Reactivity occurs against mouse c-Myb pT486 protein and the antibody is specific for the phosphorylated form of the protein.  This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. Reactivity with non-phosphorylated mouse c-Myb is minimal by ELISA and western blot. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence.  Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee, with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human, and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence.  Cross-reactivity with c-Myb from other sources has not been determined.

Attributs du produit: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The transcription factor c-Myb plays an important role in the development of all hematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesis has been first demonstrated in experiments with targeted disruption of the c-Myb gene where deletion of both alleles of the c-Myb gene was embryonally lethal due to severe anemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulates transcription of several important genes involved directly in cellular processes such as proliferation, differentiation, and apoptosis. Post-translational modifications such as phosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucial for modulation of the transactivation activity of c-Myb. Several phosphorylation sites serve to regulate c-Myb activity.

Purification: affinity purified

Stérilité: Sterile filtered

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486.
Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA and western blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb.  Specific conditions for reactivity and detection of phosphorylated c-Myb should be optimized by the end user.  Expect a band approximately ~75 kDa in size corresponding to phosphorylated c-Myb by western blotting in the appropriate cell lysate or extract.

Commentaires:

Gene Name: MYB

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.91 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Détails sur MYB

Antigène: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Autre désignation: C-MYB (MYB Produits)

Synonymes: anticorps Cmyb, anticorps c-myb, anticorps c-myb_CDS, anticorps efg, anticorps AI550390, anticorps M16449, anticorps cmyb, anticorps xmyb, anticorps MYB proto-oncogene, transcription factor, anticorps myeloblastosis oncogene, anticorps MYB proto-oncogene, transcription factor S homeolog, anticorps v-myb avian myeloblastosis viral oncogene homolog, anticorps MYB, anticorps Myb, anticorps myb.S, anticorps myb

Sujet: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. The transcription factor c-Myb plays an important role in the development of all hematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesis has been first demonstrated in experiments with targeted disruption of the c-Myb gene where deletion of both alleles of the c-Myb gene was embryonally lethal due to severe anemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulates transcription of several important genes involved directly in cellular processes such as proliferation, differentiation, and apoptosis. Post-translational modifications such as phosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucial for modulation of the transactivation activity of c-Myb. Several phosphorylation sites serve to regulate c-Myb activity.
Synonyms: Avian myeloblastosis viral (v-myb) oncogene homolog antibody, C myb antibody, MYB antibody, v-myb avian myeloblastosis viral oncogene homolog antibody

ID gène: 17863, 110556654

UniProt: P06876

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process