TNF alpha anticorps

N° du produit ABIN964798

Détail du produit anti-TNF alpha anticorps

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: This product was Protein-A purified from monospecific antiserum by chromatography. It is specific for swine TNFalpha protein. A BLAST analysis was used to suggest cross-reactivity with TNFalpha from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against TNFalpha from monkey, human, chimpanzee, macaque, baboon, vervet, mangabey, rough-toothed dolphin and beluga whale based on 94-95% homology, from manatee, dog, cat, panda, ferret, and bottle-nosed dolphin (92-93%), and horse, goat, bovine, sheep, rhinoceros, elk and nilgai based on 90-91% homology. Cross-reactivity with TNFalpha from sources other than swine has not been determined.

Attributs du produit: Tumor necrosis factor-alpha (TNFalpha) in humans is a pleiotropic inflammatory cytokine. It is expressed by many different stimulated cell types including monocytes, endothelial cells, fibroblasts and both T and B-lymphocytes, but especially by macrophages. It affects most organs. The production of TNF alpha is induced by IL-1, PDGF, IFN-beta, NGF, Oncostatin M and certain fungal, viral, and parasitic invasions. Bacterial lipopolysaccharide is an especially potent stimulus. The primary role of TNFalpha is in the regulation of immune cells. TNF alpha mediates septic shock in response to infection. It initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophages and neutrophils. TNFalpha secreted by macrophages causes blood clotting which helps contain infection. TNFalpha is also able to induce apoptotic cell death or inflammation, and to inhibit viral replication. It possesses growth stimulating, inhibitory, and self regulatory properties. Dysregulation of TNFalpha production has been implicated in a variety of human diseases, as well as cancer. The presence of TNFalpha is responsible for diverse immunomodulatory and toxic effects. For instance, TNFalpha induces neutrophil proliferation during inflammation, but it also induces neutrophil apoptosis upon binding to the TNF-R55 receptor. TNFalpha causes necrosis of some types of tumors, but promotes growth of other types. Low levels may aid in maintaining homeostasis by regulating the body's circadian rhythm, and may promote remodeling or replacement of injured and senescent tissue by stimulating fibroblast growth. High levels of TNFalpha correlate with increased risk of mortality.

Purification: purified

Immunogène: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 154 amino acids of mature swine TNFalpha.
Immunogen Type: RecombinantProtein

Isotype: IgG

Information d'application

Indications d'application: This protein A purified antibody is suitable for ELISA and Western blotting.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kDa in size corresponding to swine TNFα by western blotting in the appropriate cell lysate or extract.

Commentaires:

Gene Name: TNFA

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF alpha (TNF alpha Produits)

Synonymes: anticorps DIF, anticorps TNF-alpha, anticorps TNFA, anticorps TNFSF2, anticorps RATTNF, anticorps Tnfa, anticorps tnf, anticorps TNF-a, anticorps TNFalpha, anticorps Tnfsf1a, anticorps TNFa, anticorps cTNF, anticorps Tnf-alpha, anticorps tnfa-like, anticorps TNF-ALPHA, anticorps dif, anticorps tnfa, anticorps xtnf, anticorps tnfsf2, anticorps tnf-alpha, anticorps Cachectin, anticorps tumor necrosis factor, anticorps tumor necrosis factor b (TNF superfamily, member 2), anticorps tumor necrosis factor alpha, anticorps tumor necrosis factor a (TNF superfamily, member 2), anticorps TNF, anticorps Tnf, anticorps tnf, anticorps tnfb, anticorps tnf-alpha, anticorps LOC103694380, anticorps tnfa

Sujet: Tumor necrosis factor-alpha (TNFα) in humans is a pleiotropic inflammatory cytokine. It is expressed by many different stimulated cell types including monocytes, endothelial cells, fibroblasts and both T and B-lymphocytes, but especially by macrophages. It affects most organs. The production of TNF alpha is induced by IL-1, PDGF, IFN-beta, NGF, Oncostatin M and certain fungal, viral, and parasitic invasions. Bacterial lipopolysaccharide is an especially potent stimulus. The primary role of TNFα is in the regulation of immune cells. TNF α mediates septic shock in response to infection. It initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophages and neutrophils. TNFα secreted by macrophages causes blood clotting which helps contain infection. TNFα is also able to induce apoptotic cell death or inflammation, and to inhibit viral replication. It possesses growth stimulating, inhibitory, and self regulatory properties. Dysregulation of TNFα production has been implicated in a variety of human diseases, as well as cancer. The presence of TNFα is responsible for diverse immunomodulatory and toxic effects. For instance, TNFα induces neutrophil proliferation during inflammation, but it also induces neutrophil apoptosis upon binding to the TNF-R55 receptor. TNFα causes necrosis of some types of tumors, but promotes growth of other types. Low levels may aid in maintaining homeostasis by regulating the body's circadian rhythm, and may promote remodeling or replacement of injured and senescent tissue by stimulating fibroblast growth. High levels of TNFα correlate with increased risk of mortality.
Synonyms: TNF-A, TNF-α, TNF alpha, cachexin , cachectin; tumor necrosis factor-alpha, TNFSF2, TNFSF1A.

ID gène: 397086

NCBI Accession: NP_999187

UniProt: P23563

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome