STAT3 anticorps (pTyr705)

N° du produit ABIN964926

N° du produit (Fournisseur): 600-401-c64

Cet anticorps anti-STAT3 Polyclonal Lapin (ABIN964926) détecte spécifiquement STAT3 dans WB, IHC et ELISA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour STAT3 anticorps (pTyr705) (ABIN964926)

Antigène: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp STAT3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-STAT3 anticorps

Épitope: pTyr705

N° du produit (Fournisseur): 600-401-c64

Fournisseur: Rockland

Fonction: STAT3 phospho Y705 Antibody

Réactivité croisée (Details): Anti-STAT3 antibody is an affinity-purified antibody and is directed against the phosphorylated form of human STAT3 protein at the Y705.

Attributs du produit: Synonyms: rabbit anti-STAT3 pY705 antibody, Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, STAT3 phospho Y705, STAT3 (pTry705)

Purification: Anti-Stat3 antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified Stat3 antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids surrounding tyrosine 705 of human Stat3.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: STAT3 pY705 antibody has been tested for use in ELISA, IHC, and Western Blot. Expect a band approximately 88.1 kDa in size corresponding to phosphorylated STAT3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:10,000 - 1:30,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.9 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur STAT3

Antigène: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Autre désignation: STAT3

Sujet: Background: Signal transducer and activator of transcription 3 (Stat3) belongs to a family of cytoplasmic transcription factors that can be activated by phosphorylation by its cell surface receptor. Stat3 plays a key role in many cellular processes such as cell growth and apoptosis. It also mediates cellular responses to interleukins, KITLG/SCF,EGF, IFN-alpha and other growth factors and may mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Stat3 forms a homodimer or a heterodimer with a related family member (e.g. STAT1). Activation occurs through phosphorylation of tyrosine 705 and serine 727. Phosphorylation of Stat3 at Tyr705 induces Stat3 dimerization and nuclear translocation. Serine phosphorylation is important for stable DNA-binding of Stat3 homodimers and maximal transcriptional activity. Stat3 can have a dual role in cancer, it has been found that Stat3 protein can promote oncogenesis and have a tumor suppressor role depending upon the mutational Background of the tumor.

ID gène: 6774

NCBI Accession: NP_644805

UniProt: P40763

Pathways: Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor