H2AFX anticorps (C-Term)

N° du produit ABIN966245

Détail du produit anti-H2AFX anticorps

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp H2AFX est non-conjugé

Application: Immunohistochemistry (IHC)

Immunogène: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to Cterminal residues of human H2AFX (Histone H2A.x )

Information d'application

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour anticorps anti-H2AFX (ABIN966245)

Stiff, ODriscoll, Rief, Iwabuchi, Löbrich, Jeggo: "ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation." dans: Cancer research, Vol. 64, Issue 7, pp. 2390-6, (2004) (PubMed).

Lukas, Melander, Stucki, Falck, Bekker-Jensen, Goldberg, Lerenthal, Jackson, Bartek, Lukas: "Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention." dans: The EMBO journal, Vol. 23, Issue 13, pp. 2674-83, (2004) (PubMed).

Ward, Minn, Jorda, Chen: "Accumulation of checkpoint protein 53BP1 at DNA breaks involves its binding to phosphorylated histone H2AX." dans: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 19579-82, (2003) (PubMed).

Furuta, Takemura, Liao, Aune, Redon, Sedelnikova, Pilch, Rogakou, Celeste, Chen, Nussenzweig, Aladjem, Bonner, Pommier: "Phosphorylation of histone H2AX and activation of Mre11, Rad50, and Nbs1 in response to replication-dependent DNA double-strand breaks induced by mammalian DNA topoisomerase I cleavage complexes." dans: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 20303-12, (2003) (PubMed).

Ward, Chen: "Histone H2AX is phosphorylated in an ATR-dependent manner in response to replicational stress." dans: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 47759-62, (2001) (PubMed).

Rogakou, Boon, Redon, Bonner: "Megabase chromatin domains involved in DNA double-strand breaks in vivo." dans: The Journal of cell biology, Vol. 146, Issue 5, pp. 905-16, (1999) (PubMed).

Détails sur H2AFX

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: H2AFX (H2AFX Produits)

Synonymes: anticorps H2A.X, anticorps H2A/X, anticorps H2AX, anticorps AW228881, anticorps H2ax, anticorps Hist5-2ax, anticorps gammaH2ax, anticorps zgc:56329, anticorps h2a.x, anticorps h2a/x, anticorps h2ax, anticorps RGD1566119, anticorps h2a, anticorps h2afx, anticorps H2A histone family member X, anticorps H2A histone family, member X, anticorps H2A histone family member X L homeolog, anticorps histone cluster 1, H2ah, anticorps histone cluster 2, H2ab S homeolog, anticorps histone H2AX, anticorps H2AFX, anticorps H2afx, anticorps h2afx, anticorps h2afx.L, anticorps HIST1H2AH, anticorps hist2h2ab.S, anticorps LOC100522201, anticorps LOC100720536

Sujet: H2AFX (Histone H2A.x ) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H2AFX (Histone H2A.x ) is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2AFX interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11A, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN. Also interacts with DHX9/NDHII when phosphorylated on Ser-140. Phosphorylation at Tyr-143 (H2AXY142ph) by BAZ1B/WSTF determines the relative recruitment of either DNA repair or pro-apoptotic factors. Phosphorylation at Tyr-143 (H2AXY142ph) favors the recruitment of APBB1/FE65 and pro-apoptosis factors such as MAPK8/JNK1, triggering apoptosis. In contrast, dephosphorylation of Tyr-143 by EYA proteins (EYA1, EYA2, EYA3 or EYA4) favors the recruitment of MDC1-containing DNA repair complexes to the tail of phosphorylated Ser-140 (H2AX139ph).

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus