p21 anticorps

N° du produit ABIN967527

Cet anticorps anti-p21 est un anticorps Souris Monoclonal détectant p21 dans WB, IF, IHC (p), IP et IHC (fro). Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour p21 anticorps (ABIN967527)

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p21 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: SX118

Détail du produit anti-p21 anticorps

Marque: BD Pharmingen™

Réactivité croisée: Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Purified Human p21 Recombinant Fusion Protein

Isotype: IgG1 kappa

Information d'application

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4° C.

Références pour anticorps anti-p21 (ABIN967527)

Fredersdorf, Milne, Hall, Lu: "Characterization of a panel of novel anti-p21Waf1/Cip1 monoclonal antibodies and immunochemical analysis of p21Waf1/Cip1 expression in normal human tissues." dans: The American journal of pathology, Vol. 148, Issue 3, pp. 825-35, (1997) (PubMed).

Levine: "p53, the cellular gatekeeper for growth and division." dans: Cell, Vol. 88, Issue 3, pp. 323-31, (1997) (PubMed).

Mazars, Jat: "Expression of p24, a novel p21Waf1/Cip1/Sdi1-related protein, correlates with measurement of the finite proliferative potential of rodent embryo fibroblasts." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 1, pp. 151-6, (1997) (PubMed).

Graña, Reddy: "Cell cycle control in mammalian cells: role of cyclins, cyclin dependent kinases (CDKs), growth suppressor genes and cyclin-dependent kinase inhibitors (CKIs)." dans: Oncogene, Vol. 11, Issue 2, pp. 211-9, (1995) (PubMed).

Huppi, Siwarski, Dosik, Michieli, Chedid, Reed, Mock, Givol, Mushinski: "Molecular cloning, sequencing, chromosomal localization and expression of mouse p21 (Waf1)." dans: Oncogene, Vol. 9, Issue 10, pp. 3017-20, (1994) (PubMed).

Détails sur p21

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Autre désignation: p21

Sujet: Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) to form complexes that regulate the progression of the cell cycle. The activity of these complexes is modulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including, p16, p21, p27 and others. These proteins, referred to as inhibitors of Cdk activity (CDkIs) bind to cyclins, cdks or their complexes. p21, also known as senescent cell-derived inhibitor 1 (Sdi1), wild-type p53-activated fragment 1 (Waf1), Cdk-interacting protein 1 (Cip1), and p53-regulated inhibitor of Cdks (Pic1) inhibits cyclin D-cdk4, cyclin E-cdk3, cyclin A-cdk2, and cyclin A-cdk1. p21 can also inhibit cell cycle progression by binding to PCNA and blocking DNA replication. p21 has also shown to be a component of active cyclin-cdk complexes, suggesting that p21-containing complexes may shift between active and inactive states through changes in p21 content. Active, p21-containing complexes appear to contain one p21 molecule, whereas inactive complexes contain multiple p21 molecules. The expression of p21 can be induced in response to number of signals, including transcriptional upregulation by the tumor suppressor protein, p53. Human p21 has a calculated molecular weight of 18 kDa and runs at 21 kDa in SDS-PAGE. The epitope has been mapped to the last 20 amino acids (residues 145-164) of human p21, one of the most conserved regions between human and mouse p21. Reaction of the antibody with overlapping peptides fragments suggest that the epitope may be further mapped to residues 145-156 (TSMTDFYHSKRR). This sequence overlaps with the sequence of p21 (KRRQTSMTDFYH) which is responsible for the specific interaction of p21 with PCNA. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 21 kDa

Pathways: Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy