TNF alpha anticorps

N° du produit ABIN967569

Cité dans 4 publications. Cet anticorps anti-TNF alpha Monoclonal Hamster arménien (Clone TN3-19-12) (ABIN967569) détecte spécifiquement TNF alpha dans WB, ELISA, IP et Neut. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour TNF alpha anticorps (ABIN967569)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Clone: TN3-19-12

Détail du produit anti-TNF alpha anticorps

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogène: Recombinant mouse TNF protein

Isotype: IgG1 lambda

Information d'application

Indications d'application: 1. Neutralization:
This monoclonal antibody is useful to neutralize the bioactivity of rat TNF as tested on L929 cells at 2 x 10^5 cells/ml in cytolysis assay measured by MTT, using 12 ng/ml recombinant mouse TNF and preincubation of antibody and ligand = 60 minutes.

2. ELISA:
The purified TN3-19.12 antibody can be used as a capture antibody for a sandwich ELISA that measures TNF protein levels. For measuring mouse or rat TNF in complex fluids such as serum, ELISA Kits are recommended (refer to website).

3. IP/WB:
The TN3-19.12 antibody has been reported to be useful for immunoprecipitation and Western blotting. Please note that this application is not routinely tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-TNF alpha (ABIN967569)

Takahashi, Kapás, Fang, Krueger: "An anti-tumor necrosis factor antibody suppresses sleep in rats and rabbits." dans: Brain research, Vol. 690, Issue 2, pp. 241-4, (1996) (PubMed).

Ferran, Dautry, Mérite, Sheehan, Schreiber, Grau, Bach, Chatenoud: "Anti-tumor necrosis factor modulates anti-CD3-triggered T cell cytokine gene expression in vivo." dans: The Journal of clinical investigation, Vol. 93, Issue 5, pp. 2189-96, (1994) (PubMed).

Leiby, Fortier, Crawford, Schreiber, Nacy: "In vivo modulation of the murine immune response to Francisella tularensis LVS by administration of anticytokine antibodies." dans: Infection and immunity, Vol. 60, Issue 1, pp. 84-9, (1992) (PubMed).

Sheehan, Ruddle, Schreiber: "Generation and characterization of hamster monoclonal antibodies that neutralize murine tumor necrosis factors." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 11, pp. 3884-93, (1989) (PubMed).

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF

Sujet: The TN3-19.12 antibody reacts with rat and mouse tumor-necrosis factor (TNF) proteins (also known as TNF-alpha) . Moreover, the TN3-19.12 antibody is reported to crossreact with rabbit TNF, but it does not crossreact with mouse lymphotoxin-alpha (LT-alpha, also known as TNF-beta) nor with human TNF. The immunogen used to generate the TN3-19.12 hybridoma was E. coli-expressed, purified recombinant mouse TNF protein. This monoclonal antibody has been reported to neutralize the bioactivities of mouse, rat and rabbit TNF.

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome