Western Blotting (WB), ELISA, Immunoprecipitation (IP), Neutralization (Neut)
Marque
BD Pharmingen™
Attributs du produit
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results. 2. Please refer to us for technical protocols. 3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Stérilité
0.2 μm filtered
niveau d'endotoxine
Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
1. Neutralization: This monoclonal antibody is useful to neutralize the bioactivity of rat TNF as tested on L929 cells at 2 x 10^5 cells/ml in cytolysis assay measured by MTT, using 12 ng/ml recombinant mouse TNF and preincubation of antibody and ligand = 60 minutes.
2. ELISA: The purified TN3-19.12 antibody can be used as a capture antibody for a sandwich ELISA that measures TNF protein levels. For measuring mouse or rat TNF in complex fluids such as serum, ELISA Kits are recommended (refer to website).
3. IP/WB: The TN3-19.12 antibody has been reported to be useful for immunoprecipitation and Western blotting. Please note that this application is not routinely tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Buffer
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Agent conservateur
Azide free
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Takahashi, Kapás, Fang, Krueger: "An anti-tumor necrosis factor antibody suppresses sleep in rats and rabbits." dans: Brain research, Vol. 690, Issue 2, pp. 241-4, (1996) (PubMed).
Ferran, Dautry, Mérite, Sheehan, Schreiber, Grau, Bach, Chatenoud: "Anti-tumor necrosis factor modulates anti-CD3-triggered T cell cytokine gene expression in vivo." dans: The Journal of clinical investigation, Vol. 93, Issue 5, pp. 2189-96, (1994) (PubMed).
Leiby, Fortier, Crawford, Schreiber, Nacy: "In vivo modulation of the murine immune response to Francisella tularensis LVS by administration of anticytokine antibodies." dans: Infection and immunity, Vol. 60, Issue 1, pp. 84-9, (1992) (PubMed).
Sheehan, Ruddle, Schreiber: "Generation and characterization of hamster monoclonal antibodies that neutralize murine tumor necrosis factors." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 11, pp. 3884-93, (1989) (PubMed).
The TN3-19.12 antibody reacts with rat and mouse tumor-necrosis factor (TNF) proteins (also known as TNF-alpha) . Moreover, the TN3-19.12 antibody is reported to crossreact with rabbit TNF, but it does not crossreact with mouse lymphotoxin-alpha (LT-alpha, also known as TNF-beta) nor with human TNF. The immunogen used to generate the TN3-19.12 hybridoma was E. coli-expressed, purified recombinant mouse TNF protein. This monoclonal antibody has been reported to neutralize the bioactivities of mouse, rat and rabbit TNF.