EGFR anticorps (AA 1020-1046)

N° du produit ABIN967697

L’anticorps Souris Monoclonal anti-EGFR a été validé pour WB, IHC, IF, FACS et IP. Il convient pour détecter EGFR dans des échantillons de Humain et Souris. Il y a 5+ publications disponibles.

Aperçu rapide pour EGFR anticorps (AA 1020-1046) (ABIN967697)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFR est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 13-EGFR

Détail du produit anti-EGFR anticorps

Épitope: AA 1020-1046

Réactivité croisée: Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human EGF Receptor aa. 1020-1046

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-EGFR (ABIN967697)

Deugnier, Faraldo, Janji, Rousselle, Thiery, Glukhova: "EGF controls the in vivo developmental potential of a mammary epithelial cell line possessing progenitor properties." dans: The Journal of cell biology, Vol. 159, Issue 3, pp. 453-63, (2002) (PubMed).

Golubovskaya, Beviglia, Xu, Earp, Craven, Cance: "Dual inhibition of focal adhesion kinase and epidermal growth factor receptor pathways cooperatively induces death receptor-mediated apoptosis in human breast cancer cells." dans: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38978-87, (2002) (PubMed).

Ford, Valle, Soroka, Merchant: "EGF receptor activation stimulates endogenous gastrin gene expression in canine G cells and human gastric cell cultures." dans: The Journal of clinical investigation, Vol. 99, Issue 11, pp. 2762-71, (1997) (PubMed).

Kizaka-Kondoh, Matsuda, Okayama: "CrkII signals from epidermal growth factor receptor to Ras." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 22, pp. 12177-82, (1996) (PubMed).

Ward, Gough, Rashke, Wan, Tribbick, Wang: "Systematic mapping of potential binding sites for Shc and Grb2 SH2 domains on insulin receptor substrate-1 and the receptors for insulin, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor." dans: The Journal of biological chemistry, Vol. 271, Issue 10, pp. 5603-9, (1996) (PubMed).

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGF Receptor

Sujet: Epidermal Growth Factor (EGF) elicits a variety of cellular responses that are initiated by EGF receptor (EGF-R) binding and activation of intrinsic tyrosine kinase activity. Following ligand binding, EGF receptor is autophosphorylated and, in turn, phosphorylates several other endogenous proteins including phospholipase Cgamma. These events initiate a number of intracellular responses that include increased levels of intracellular Ca2+ and transient expression of the nuclear oncogene products c-Myc and c-Fos. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 180 kDa

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins