SOS1 anticorps (AA 1-109)

N° du produit ABIN967741

Cet anticorps Souris Monoclonal détecte spécifiquement SOS1 dans WB, IF, IP et IHC. Il présente une réactivité envers Humain, Souris, Rat et Chien et a été mentionné dans 5+ publications.

Aperçu rapide pour SOS1 anticorps (AA 1-109) (ABIN967741)

Antigène: SOS1 (Son of Sevenless Homolog 1 (SOS1))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SOS1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Clone: 25-SOS1

Détail du produit anti-SOS1 anticorps

Épitope: AA 1-109

Réactivité croisée: Rat (Rattus), Chien, Humain

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse mSos1 aa. 1-109

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968539, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-SOS1 (ABIN967741)

Furuta, Miura, Copeland, Shang, Oshima, Kamata: "Light Chain 3 associates with a Sos1 guanine nucleotide exchange factor: its significance in the Sos1-mediated Rac1 signaling leading to membrane ruffling." dans: Oncogene, Vol. 21, Issue 46, pp. 7060-6, (2002) (PubMed).

Kardinal, Konkol, Lin, Eulitz, Schmidt, Estrov, Talpaz, Arlinghaus, Feller: "Chronic myelogenous leukemia blast cell proliferation is inhibited by peptides that disrupt Grb2-SoS complexes." dans: Blood, Vol. 98, Issue 6, pp. 1773-81, (2001) (PubMed).

Salojin, Zhang, Meagher, Delovitch: "ZAP-70 is essential for the T cell antigen receptor-induced plasma membrane targeting of SOS and Vav in T cells." dans: The Journal of biological chemistry, Vol. 275, Issue 8, pp. 5966-75, (2000) (PubMed).

Buday, Downward: "Epidermal growth factor regulates p21ras through the formation of a complex of receptor, Grb2 adapter protein, and Sos nucleotide exchange factor." dans: Cell, Vol. 73, Issue 3, pp. 611-20, (1993) (PubMed).

Egan, Giddings, Brooks, Buday, Sizeland, Weinberg: "Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation." dans: Nature, Vol. 363, Issue 6424, pp. 45-51, (1993) (PubMed).

Détails sur SOS1

Antigène: SOS1 (Son of Sevenless Homolog 1 (SOS1))

Autre désignation: Sos1

Sujet: The Sos (son of sevenless) gene was originally identified in Drosophila, and two mammalian homologues (mSos1 and mSos2) were isolated from a mouse cDNA library. These two cDNAs predict proteins that are approximately 70% identical in their amino acid residues. Both mSos1 and mSos2 are expressed in a wide number of mouse embryonic and adult tissues as well as in several cell lines. The human homologues, hSos1 and hSos2 have also been isolated and show a very high degree of amino acid identity to the mouse genes (98% for Sos1). Sos1 has a predicted molecular weight of 150kDa, but the apparent molecular weight is closer to 170 kDa, presumably due to a high proline content. The mammalian Sos1 protein has a highly specific guanine nucleotide exchange activity toward p21ras . In EGF-stimulated cells, Sos1 interacts with the SH3 domains of GRB2, and GRB2 binds via its SH2 domain to tyrosine 1068 of the activated EGF receptor. Thus, GRB2 recruits Sos1 to the plasma membrane and enables it to activate the Ras signaling pathway by enhancing GTP loading on p21ras .

Poids moléculaire: 170 kDa

Pathways: Signalisation RTK, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling