PKC beta anticorps (AA 126-324)

N° du produit ABIN967769

L’anticorps Souris Monoclonal anti-PKC beta a été validé pour WB, IHC, IF et IP. Il convient pour détecter PKC beta dans des échantillons de Humain, Rat, Souris et Poulet. Il y a 5+ publications disponibles.

Aperçu rapide pour PKC beta anticorps (AA 126-324) (ABIN967769)

Antigène: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Reactivité: Humain, Rat, Souris, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PKC beta est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 36-PKCb

Détail du produit anti-PKC beta anticorps

Épitope: AA 126-324

Réactivité croisée: Rat (Rattus), Souris, Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human PKCbeta aa. 126-324

Isotype: IgG2b

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-PKC beta (ABIN967769)

Masur, Lang, Niggemann, Zanker, Entschladen: "High PKC alpha and low E-cadherin expression contribute to high migratory activity of colon carcinoma cells." dans: Molecular biology of the cell, Vol. 12, Issue 7, pp. 1973-82, (2001) (PubMed).

Stebbins, Mochly-Rosen: "Binding specificity for RACK1 resides in the V5 region of beta II protein kinase C." dans: The Journal of biological chemistry, Vol. 276, Issue 32, pp. 29644-50, (2001) (PubMed).

Bell, Burns: "Lipid activation of protein kinase C." dans: The Journal of biological chemistry, Vol. 266, Issue 8, pp. 4661-4, (1991) (PubMed).

Soderling: "Protein kinases. Regulation by autoinhibitory domains." dans: The Journal of biological chemistry, Vol. 265, Issue 4, pp. 1823-6, (1990) (PubMed).

Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." dans: Nature, Vol. 334, Issue 6184, pp. 661-5, (1988) (PubMed).

Détails sur PKC beta

Antigène: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Autre désignation: PKC beta

Sujet: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members ( delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. PKCbeta is highly expressed in brain and hematopoietic cells. Autophosphorylation of PKCbeta occurs at the N- and C-terminal regions, as well as within the hinge region. However, only the COOH- terminal autophosphorylation sites are essential for PKCbeta's function and subcellular localization. PKCbeta is critical for the proliferation of K562 cells, as well as being an important regulator of human melanogenesis.

Poids moléculaire: 80 kDa

Pathways: Signalisation WNT, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling