ROCK2 anticorps (AA 567-718)

N° du produit ABIN968071

Détail du produit anti-ROCK2 anticorps

Antigène: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Épitope: AA 567-718

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ROCK2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Réactivité croisée: Souris, Chien, Humain

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat ROKalpha aa. 567-718

Clone: 21-ROCK

Isotype: IgG1

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-ROCK2 (ABIN968071)

Begum, Sandu, Ito, Lohmann, Smolenski: "Active Rho kinase (ROK-alpha ) associates with insulin receptor substrate-1 and inhibits insulin signaling in vascular smooth muscle cells." dans: The Journal of biological chemistry, Vol. 277, Issue 8, pp. 6214-22, (2002) (PubMed).

Wang, Eto, Steers, Somlyo, Somlyo: "RhoA-mediated Ca2+ sensitization in erectile function." dans: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 30614-21, (2002) (PubMed).

Adachi, Vita, Sannohe, Stafford, Alam, Kayaba, Chihara: "The functional role of rho and rho-associated coiled-coil forming protein kinase in eotaxin signaling of eosinophils." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4609-15, (2001) (PubMed).

Sahai, Olson, Marshall: "Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility." dans: The EMBO journal, Vol. 20, Issue 4, pp. 755-66, (2001) (PubMed).

Leung, Manser, Tan, Lim: "A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to peripheral membranes." dans: The Journal of biological chemistry, Vol. 270, Issue 49, pp. 29051-4, (1996) (PubMed).

Détails sur ROCK2

Antigène: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Autre désignation: ROCK-II (ROCK2 Produits)

Synonymes: anticorps rocka-a, anticorps xROK, anticorps rock2, anticorps rok2, anticorps ROCK2, anticorps B230113H15Rik, anticorps ROKalpha, anticorps Rho-kinase, anticorps Rock-II, anticorps Rock2m, anticorps mKIAA0619, anticorps ROCK-II, anticorps ROK, anticorps Rho-associated, coiled-coil containing protein kinase 2 L homeolog, anticorps rho-associated, coiled-coil containing protein kinase 2a, anticorps Rho associated coiled-coil containing protein kinase 2, anticorps Rho-associated, coiled-coil containing protein kinase 2, anticorps Rho-associated coiled-coil containing protein kinase 2, anticorps rock2.L, anticorps rock2a, anticorps ROCK2, anticorps Rock2

Sujet: Activity of the GTP-binding proteins is regulated by GAPs, that accelerate binding, and GTPases, that enhance the rate of GTP hydrolysis (Ras, Rho, cdc42Hs, and Rac). The isoforms of Rho, a small GTP-binding protein, regulate cellular processes such as the formation of the stress fibers, lamelipodia, and filopodia. ROKalpha (RhoA-binding kinase) is a Ser/Thr protein kinase that interects with the GTP-binding form of RhoA. Like RhoA, ROKalpha localizes at the cellular margins and colocalizes with acti filaments. The ROKalpha gene encodes a protein of 1302 amino acids with homology to the human myotonic kinase. Although ROKalpha binds to GTP-RhoA, it is not yet clear whether this interaction induces the kinase activity of ROKalpha.
Synonyms: ROKalpha

Poids moléculaire: 180 kDa

Pathways: Dynamique des Microtubules, Signalisation WNT, Tube Formation