CENPF anticorps (AA 209-381)

N° du produit ABIN968167

Cet anticorps Souris Monoclonal détecte spécifiquement CENPF dans IHC, WB, IP et IF. Il présente une réactivité envers Humain et a été mentionné dans 5+ publications.

Aperçu rapide pour CENPF anticorps (AA 209-381) (ABIN968167)

Antigène: CENPF (Centromere Protein F (CENPF))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CENPF est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 11-Mitosin

Détail du produit anti-CENPF anticorps

Épitope: AA 209-381

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Mitosin aa. 209-381

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-CENPF (ABIN968167)

Trinkle-Mulcahy, Andrews, Wickramasinghe, Sleeman, Prescott, Lam, Lyon, Swedlow, Lamond: "Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle." dans: Molecular biology of the cell, Vol. 14, Issue 1, pp. 107-17, (2003) (PubMed).

Landberg, Erlanson, Roos, Tan, Casiano: "Nuclear autoantigen p330d/CENP-F: a marker for cell proliferation in human malignancies." dans: Cytometry, Vol. 25, Issue 1, pp. 90-8, (1997) (PubMed).

Casiano, Humbel, Peebles, Covini, Tan: "Autoimmunity to the cell cycle-dependent centromere protein p330d/CENP-F in disorders associated with cell proliferation." dans: Journal of autoimmunity, Vol. 8, Issue 4, pp. 575-86, (1996) (PubMed).

Zhu, Mancini, Chang, Liu, Chen, Shan, Jones, Yang-Feng, Lee: "Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression." dans: Molecular and cellular biology, Vol. 15, Issue 9, pp. 5017-29, (1995) (PubMed).

Rattner, Rao, Fritzler, Valencia, Yen: "CENP-F is a .ca 400 kDa kinetochore protein that exhibits a cell-cycle dependent localization." dans: Cell motility and the cytoskeleton, Vol. 26, Issue 3, pp. 214-26, (1994) (PubMed).

Détails sur CENPF

Antigène: CENPF (Centromere Protein F (CENPF))

Autre désignation: Mitosin

Sujet: Mitosin, a nuclear proetin of 3113 amino acids, contains a pair of tandem repeats and seven leucine repeats. In vitro, Mitosin directly interacts with the retinoblastoma protein, Rb. Mitosin is expressed throughout the cell cycle, with levels being lowest during G1, and it localizes at the kinetochore during the mitotic phase. The subcellular redistribution of Mitosin to the kinetochore is linked to its phosphorylation. Its expression is linked to patients with autoimmune diseases characterized by abnormal cell proliferation. Ectopic expression of a truncated version of Mitosin blocks the progression of the cell cycle. This suggests that the protein has an important role during cell proliferation. Because of similarities in subcellular localization and expression, Mitosin and the autoantigen p300/CENP-F, which is also linked to a multitude of autoimmune disorders, are probably the same protein.

Poids moléculaire: 357 kDa

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2