Golgin A2 (GOLGA2) (AA 869-982) anticorps

N° du produit ABIN968197

Détail du produit

Antigène: Golgin A2 (GOLGA2)

Épitope: AA 869-982

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Humain, Chien, Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat GM130 aa. 869-982

Clone: 35-GM130

Isotype: IgG1 kappa

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Référence

Ireton, Davis, van Hengel, Mariner, Barnes, Thoreson, Anastasiadis, Matrisian, Bundy, Sealy, Gilbert, van Roy, Reynolds: "A novel role for p120 catenin in E-cadherin function." dans: The Journal of cell biology, Vol. 159, Issue 3, pp. 465-76, (2002) (PubMed).

Marra, Maffucci, Daniele, Tullio, Ikehara, Chan, Luini, Beznoussenko, Mironov, De Matteis: "The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment." dans: Nature cell biology, Vol. 3, Issue 12, pp. 1101-13, (2002) (PubMed).

Perez, Pernet-Gallay, Nizak, Goodson, Kreis, Goud: "CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family." dans: The Journal of cell biology, Vol. 156, Issue 4, pp. 631-42, (2002) (PubMed).

Ralston, Lu, Ploug: "The organization of the Golgi complex and microtubules in skeletal muscle is fiber type-dependent." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 24, pp. 10694-705, (2000) (PubMed).

Nakamura, Lowe, Levine, Rabouille, Warren: "The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner." dans: Cell, Vol. 89, Issue 3, pp. 445-55, (1997) (PubMed).

Détail du antigène

Antigène: Golgin A2 (GOLGA2)

Autre désignation: GM130 (GOLGA2 Produits)

Synonymes: anticorps gm130, anticorps MGC81213, anticorps AW555139, anticorps GM130, anticorps mKIAA4150, anticorps Gm130, anticorps golgin A2 S homeolog, anticorps golgin A2, anticorps golgi autoantigen, golgin subfamily a, 2, anticorps golga2.S, anticorps GOLGA2, anticorps Golga2

Sujet: Maturation and post-translational modification of proteins occurs after their biosynthesis at the endoplasmic reticulum and their transport through the Golgi apparatus. The process involves the transport of vesicles carrying the proteins through a vectorial process of vesicle budding and fusion from the cis-compartment to the medial-compartment and the trans-compartment of the Golgi apparatus. The detergent insoluble fraction of the Golgi is named matrix and is required for proper morphology of the Golgi membranes. GM130 (Golgi matrix protein of 130 kDa) is a protein isolated from the Triton™ X-100-insoluble Golgi matrix and peripherally associated with the cis-compartment, as demonstrated by co-localization with syntaxin5. GM130 is homologous to the Golgi autoantigen golgin 95. GM130 interacts through its N-terminal domain with p115 and with the Golgi membranes at the C-terminal portion. Furthermore, the mitotic phosphorylation of GM130 blocks the interaction with p115. Thus, GM130 appears to function as a structural element of the Golgi apparatus that also provides attachment sites for membranes and other Golgi proteins. The 35/GM130 monoclonal antibody recognizes GM130, regardless of phosphorylation status.

Poids moléculaire: 130 kDa

Pathways: SARS-CoV-2 Protein Interactome