SLC9A3R1 anticorps (AA 128-249)

N° du produit ABIN968397

Détail du produit anti-SLC9A3R1 anticorps

Antigène: SLC9A3R1 (Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1 (SLC9A3R1))

Épitope: AA 128-249

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SLC9A3R1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human EBP50 aa. 128-249

Clone: 6-EBP50

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-SLC9A3R1 (ABIN968397)

Glynne, Darling, Picot, Evans: "Epithelial inducible nitric-oxide synthase is an apical EBP50-binding protein that directs vectorial nitric oxide output." dans: The Journal of biological chemistry, Vol. 277, Issue 36, pp. 33132-8, (2002) (PubMed).

Bretscher, Reczek, Berryman: "Ezrin: a protein requiring conformational activation to link microfilaments to the plasma membrane in the assembly of cell surface structures." dans: Journal of cell science, Vol. 110 ( Pt 24), pp. 3011-8, (1998) (PubMed).

Reczek, Berryman, Bretscher: "Identification of EBP50: A PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family." dans: The Journal of cell biology, Vol. 139, Issue 1, pp. 169-79, (1998) (PubMed).

Reczek, Bretscher: "The carboxyl-terminal region of EBP50 binds to a site in the amino-terminal domain of ezrin that is masked in the dormant molecule." dans: The Journal of biological chemistry, Vol. 273, Issue 29, pp. 18452-8, (1998) (PubMed).

Détails sur SLC9A3R1

Antigène: SLC9A3R1 (Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1 (SLC9A3R1))

Autre désignation: EBP50 (SLC9A3R1 Produits)

Synonymes: anticorps EBP50, anticorps NHERF, anticorps NHERF-1, anticorps NHERF1, anticorps NPHLOP2, anticorps SLC9A3R1, anticorps cb895, anticorps slc9a3r2, anticorps wu:fd20a02, anticorps zgc:55541, anticorps zgc:77629, anticorps EBP-50, anticorps NHE-RF, anticorps nherf, anticorps nherf-1, anticorps nherf1, anticorps slc9a3r1, anticorps SLC9A3 regulator 1, anticorps microRNA 3615, anticorps solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1a, anticorps solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1, anticorps SLC9A3 regulator 1 S homeolog, anticorps SLC9A3R1, anticorps MIR3615, anticorps slc9a3r1a, anticorps Slc9a3r1, anticorps slc9a3r1.S

Sujet: Integral plasma membrane proteins are stabilized by linkages to the cortical actin cytoskeleton, which structurally supports the membrane and contributes to processes such as endocytosis, exocytosis, and transmembrane signaling. The ERM (ezrin-radixin-moesin) family of proteins provides these structural linkages. The ERM proteins contain a 300-residue N-terminal domain, a 170-residue alpha-helical region, and a C-terminal 100-residue domain that contains F-actin binding sites. The N-terminal domain interacts with the cytoplasmic domain of CD44, the regulatory subunit of PKA, and the PDZ domain containing ERM-binding phosphoprotein (EBP-50). In polarized epithelial cells, EBP-50 links ezrin and the cytoplasmic regions of transmembrane proteins such as the cystic fibrosis transmembrane conductance regulator (CFTR) and the beta2-adrenergic receptor. EBP50 contains two PDZ domains followed by a C-terminal tail. It colocalizes with ezrin in apical microvilli and is also thought to interact with the Na+-H+ exchanger NHE3 to confer cAMP-mediated inhibition of Na+-H+ exchange. Thus, EBP-50 mediates membrane attachment to the cytoskeleton and may also function in the regulation of ion exchange. This antibody is routinely tested in western blot analysis.

Poids moléculaire: 50-53 kDa

Pathways: Proton Transport, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, SARS-CoV-2 Protein Interactome