XRCC5 anticorps (AA 103-315)

N° du produit ABIN968493

Détail du produit anti-XRCC5 anticorps

Antigène: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Épitope: AA 103-315

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp XRCC5 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Ku80 aa. 103-315

Clone: 7-Ku80

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-XRCC5 (ABIN968493)

Kim, Kim, Han, Jeong, Chung, Kang, Li: "Ku autoantigen affects the susceptibility to anticancer drugs." dans: Cancer research, Vol. 59, Issue 16, pp. 4012-7, (1999) (PubMed).

Majumder, Ghoshal, Li, Jacob: "Hypermethylation of metallothionein-I promoter and suppression of its induction in cell lines overexpressing the large subunit of Ku protein." dans: The Journal of biological chemistry, Vol. 274, Issue 40, pp. 28584-9, (1999) (PubMed).

Smith, Jackson: "The DNA-dependent protein kinase." dans: Genes & development, Vol. 13, Issue 8, pp. 916-34, (1999) (PubMed).

Détails sur XRCC5

Antigène: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Autre désignation: Ku80 (XRCC5 Produits)

Synonymes: anticorps AI314015, anticorps Ku80, anticorps Ku86, anticorps Kup80, anticorps KARP-1, anticorps KARP1, anticorps KU80, anticorps KUB2, anticorps NFIV, anticorps xrcc5-a, anticorps XRCC5, anticorps X-ray repair cross complementing 5, anticorps X-ray repair complementing defective repair in Chinese hamster cells 5, anticorps X-ray repair complementing defective repair in Chinese hamster cells 5 L homeolog, anticorps XRCC5, anticorps Xrcc5, anticorps xrcc5.L

Sujet: DNA-dependent protein kinase (DNA-PK) is a trimeric enzyme that contains a catalytic subunit of 350 kDa (DNA-PKcs) and a heterodimeric regulatory subunit of 70 kDa (Ku70) and 86 kDa (Ku80). DNA-PKcs is inactive alone and depends on the regulatory subunit for subcellular localization and kinase activity. The DNA-PKcs/Ku70/Ku80 complex is involved in V(D)J recombination and DNA double-stranded break repair. Ku70 and Ku80 are abundant nuclear DNA-binding proteins. Besides functioning in a complex with DNA-PKcs, Ku proteins may act in multiple cellular processes including transcriptional regulation, ATPase and helicase activity, alteration in chromatin structure, cell cycle regulation, and maintenance of telomere length. In rat fibroblasts, Ku80 overexpression leads to hypermethylation and silencing of metallothionein gene expression. Ku70-/- and Ku80-/- cells are less resistant to anticancer drug-induced apoptosis, suggesting a role for Ku proteins in the prevention of apoptotic signaling. Thus, Ku70 and Ku80 may have multiple roles during DNA transcription, repair, and maintenance depending on the protein interactions that are involved.

Poids moléculaire: 80 kDa

Pathways: Réparation de l'ADN