NEDD4 anticorps (AA 173-262)

N° du produit ABIN968557

Détail du produit anti-NEDD4 anticorps

Antigène: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Épitope: AA 173-262

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEDD4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Nedd4 aa. 173-262

Clone: 15-Nedd4

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-NEDD4 (ABIN968557)

Peruzzi, Prisco, Morrione, Valentinis, Baserga: "Anti-apoptotic signaling of the insulin-like growth factor-I receptor through mitochondrial translocation of c-Raf and Nedd4." dans: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 25990-6, (2001) (PubMed).

Harvey, Dinudom, Komwatana, Jolliffe, Day, Parasivam, Cook, Kumar: "All three WW domains of murine Nedd4 are involved in the regulation of epithelial sodium channels by intracellular Na+." dans: The Journal of biological chemistry, Vol. 274, Issue 18, pp. 12525-30, (1999) (PubMed).

Plant, Yeger, Staub, Howard, Rotin: "The C2 domain of the ubiquitin protein ligase Nedd4 mediates Ca2+-dependent plasma membrane localization." dans: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32329-36, (1998) (PubMed).

Hatakeyama, Jensen, Weissman: "Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases." dans: The Journal of biological chemistry, Vol. 272, Issue 24, pp. 15085-92, (1997) (PubMed).

Détails sur NEDD4

Antigène: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Autre désignation: Nedd4 (NEDD4 Produits)

Synonymes: anticorps NEDD4-1, anticorps RPF1, anticorps AA959633, anticorps AL023035, anticorps AU019897, anticorps E430025J12Rik, anticorps Nedd4-1, anticorps Nedd4a, anticorps mKIAA0093, anticorps CG32184, anticorps CG42279, anticorps CG7555, anticorps DNedd4, anticorps Dmel\\CG42279, anticorps Dmel_CG32184, anticorps Dmel_CG7555, anticorps dNedd4, anticorps dNedd4-1, anticorps nedd4, anticorps GB16853, anticorps NEDD4, anticorps Nedd4, anticorps neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase, anticorps neural precursor cell expressed, developmentally down-regulated 4, anticorps CG42279 gene product from transcript CG42279-RJ, anticorps E3 ubiquitin-protein ligase Nedd-4, anticorps E3 ubiquitin-protein ligase NEDD4, anticorps NEDD4, anticorps Nedd4, anticorps LOC411723, anticorps LOC100457150, anticorps nedd4, anticorps LOC100547485

Sujet: Regulated protein degradation involves conjugation of ubiquitin to proteins for degradation by the 26S proteasome. Protein ubiquitination is the end product of a multienzyme cascade that involves sequential thiol-ester bond interactions between ubiquitin and enzymes termed E1-E3. Neuronal precursor cell-expressed developmentally down-regulated 4 (Nedd4) is a multimodular ubiquitin protein ligase E3 that contains a Ca 2+-lipid binding domain (CaLB), 3 WW domains, and a C-terminal ubiquitin protein ligase hect domain. The CaLB is characteristic of C2 domain-containing proteins, such as PKC, phospholipase C, phospholipase A2, and synaptogamin. Nedd4 is expressed differentially in the fetal and adult brain and its highest expression is in lung and kidney. Although it localizes in the cytoplasm, increased intracellular Ca 2+ induces plasma membrane association of Nedd4. In addition, increases in intracellular Na+ leads to Nedd4 binding to epithelial Na+ channels via its WW domains, which results in targeting of Na+ channels for degradation. Thus, the subcellular localization and protein interactions of Nedd4 are regulated in a Ca2+- and Na+-dependent manner. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 110 kDa

Pathways: Signalisation Notch, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2