XRCC4 anticorps (AA 53-168)

N° du produit ABIN968566

Détail du produit anti-XRCC4 anticorps

Antigène: XRCC4 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4))

Épitope: AA 53-168

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp XRCC4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human XRCC4 aa. 53-168

Clone: 4-XRCC4

Isotype: IgG2b

Information d'application

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-XRCC4 (ABIN968566)

Gao, Sun, Frank, Dikkes, Fujiwara, Seidl, Sekiguchi, Rathbun, Swat, Wang, Bronson, Malynn, Bryans, Zhu, Chaudhuri, Davidson, Ferrini, Stamato, Orkin, Greenberg, Alt: "A critical role for DNA end-joining proteins in both lymphogenesis and neurogenesis." dans: Cell, Vol. 95, Issue 7, pp. 891-902, (1999) (PubMed).

Modesti, Hesse, Gellert: "DNA binding of Xrcc4 protein is associated with V(D)J recombination but not with stimulation of DNA ligase IV activity." dans: The EMBO journal, Vol. 18, Issue 7, pp. 2008-18, (1999) (PubMed).

Li, Otevrel, Gao, Cheng, Seed, Stamato, Taccioli, Alt: "The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)J recombination." dans: Cell, Vol. 83, Issue 7, pp. 1079-89, (1996) (PubMed).

Détails sur XRCC4

Antigène: XRCC4 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4))

Autre désignation: XRCC4 (XRCC4 Produits)

Synonymes: anticorps zgc:73312, anticorps MGC81443, anticorps homolog of human DNA ligase iv-binding protein XRCC4, anticorps 2310057B22Rik, anticorps AW413319, anticorps AW545101, anticorps X-ray repair cross complementing 4, anticorps X-ray repair complementing defective repair in Chinese hamster cells 4, anticorps X-ray repair complementing defective repair in Chinese hamster cells 4 L homeolog, anticorps DNA ligase IV-binding protein, anticorps XRCC4, anticorps xrcc4, anticorps xrcc4.L, anticorps Xrcc4

Sujet: DNA double-strand breaks (DSB) are generated during intrinsic eukaryotic DNA recombination events such as assembly of antigen receptor genes and meiotic and mitotic recombination. DSB repair proteins are also required to repair breaks induced by extrinsic factors such as ionizing radiation and mutagenic chemicals. DNA-PKcs, Ku70/Ku80, DNA ligase IV, and X-Ray Cross Complementation group 4 (XRCC4) are DSB proteins involved in both V(D)J recombination and DNA double-stranded break repair. XRCC4 activates DNA ligase IV and cells deficient in XRCC4 inefficiently form coding joints and signal joints during V(D)J recombination. XRCC4 contains a C-terminal nuclear localization sequence (NLS) and multiple phosphorylation sites, binds DNA, and is an effective substrate for DNA-PK. Phosphorylation of XRCC4 has no effect on its interactions with DNA ligase IV or end-joining activity, but can inhibit its DNA binding activity. Mice deficient in XRCC4 exhibit defects in lymphogenesis and apoptotic death of postmitotic neurons during neurogenesis. Thus, XRCC4 is a ubiquitous protein involved in DNA end joining during DNA recombination and repair, which is critical for cell growth and survival.
Synonyms: X-Ray Cross Complementation group 4

Poids moléculaire: 55 kDa

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response