Dynamin Associated Protein 160 (DAP160) (AA 800-909) anticorps

N° du produit ABIN968598

Détail du produit

Antigène: Dynamin Associated Protein 160 (DAP160)

Épitope: AA 800-909

Reactivité: Poulet, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Application: Immunofluorescence (IF), Western Blotting (WB)

Réactivité croisée: Rat (Rattus), Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Ese-1 aa. 800-909

Clone: 29-Intersectin

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Référence

Okamoto, Schoch, Südhof: "EHSH1/intersectin, a protein that contains EH and SH3 domains and binds to dynamin and SNAP-25. A protein connection between exocytosis and endocytosis?" dans: The Journal of biological chemistry, Vol. 274, Issue 26, pp. 18446-54, (1999) (PubMed).

Sengar, Wang, Bishay, Cohen, Egan: "The EH and SH3 domain Ese proteins regulate endocytosis by linking to dynamin and Eps15." dans: The EMBO journal, Vol. 18, Issue 5, pp. 1159-71, (1999) (PubMed).

Détail du antigène

Antigène: Dynamin Associated Protein 160 (DAP160)

Autre désignation: Intersectin

Sujet: ESE-1 (epithelium-specific, EH domain and SH3 domain regulator of endocytosis) contains two N-terminal EH (Ep15 homology) domains, a central coiled-coil domain, and five C-terminal SH3 domains. ESE-1 is also known as the adaptor protein Intersectin. Intersectin constitutively associates with Eps15 to form a complex that is analogous to the yeast Pan1p-End3p complex, which is required for endocytosis and organization of the actin cytoskeleton. The EH, coiled-coil, and SH3 domains of intersectin bind epsin family proteins, SNAP25 and SNAP23, and dynamin, respectively. Thus, intersectin functions in complex with Eps15 to regulate endocytosis by linking components of the endocytic machinery.

Poids moléculaire: 140/200 kDa